Journal of Hebei Medical University

Previous Articles     Next Articles

Study on the efficiencies of the mobilization， collection and the safety of the transplantation of autologous peripheral blood stem cell for myocardial infarction patients

  

  1. 1.Department of Cardiac Surgery, the Chest Hosipital of Hebei Province, Shijiazhuang 050041, China；
    2.Department of Cardiology, the Chest Hosipital of Hebei Province, Shijiazhuang
    050041, China; 3.Department of Chest Diseases I, the Chest Hosipital of Hebei Province, Shijiazhuang 050041, China
  • Online:2016-05-25 Published:2016-05-20

PDF (PC)

206

Abstract: [
Abstract ] Objective Toinvestigatetheefficienciesof mobilization andcollection of
autologousperipheralbloodstemcell ( APBSC ) formyocardialinfarctionpatients.Andtostudy
thesafetyandfeasibilityofmyocardialinfarctiontreatmentviatheautologousperipheralblood
stemcelltransplantation ( APBSCT ) .Methods Forty-sixmyocardialinfarctionpatientswiththe herapyofconventionaldrugweregivenrecombinanthumangranulocytecolonystimulatingfactor
(
rhG-CSF ) 450 μ g / d ( ≤65kg ) or600 μ g / d ( >65kg ) bysubcutaneousinjectionatfivedays
beforecoronaryarterybypassgraft ( CABG ), accordingtopatient'sbodymass , thenwerecarried
onthe APBSC mobilizationandexpansion.APBSCs werecollected24hbeforeCABG.All
patientsunderwentCABGwithnon-cardiopulmonaryby-pass , andtheAPBSCswereinjectedvia
thebridgecoronary vesselsandsub-epicardially.Beforeandafter APBSC mobilizationand
collection , mononuclearcell ( MNC ) andCD34
+
cellfromtheperipheralbloodweretested , and
thebloodpressure , heartrate , respiratory , whitebloodcell ( WBC ), redbloodcell ( RBC ),
hemoglobin ( Hb ), hematocrit ( Hct ), platelet ( Plt ) andserum Ca
2+
werealsotested.Adverse
reactionsintheprocessofAPBSC mobilization , collectionandtransplantation wereobserved.
Results ThenumbersofWBC , MNCandCD34
+
cellsinperipheralbloodwereallsignificantly
increased ( P <0.01 ) after APBSC mobilizationthanthatbefore APBSC mobilization.After
APBSCcollection , thenumbersofMNCandCD34
+ cellsweresignificantlyincreased (
P <0.01 ) .
Nosignificanceexistedinthebloodpressure , heartrate , respiratory , WBC , RBC , Hb , Hctand
serum Ca
2+
( P >0.05 ) .ThenumberofPlt wassignificantly ( P <0.01 ) decreasedafter
collection.Adversereactionsoccurredin20casesduring APBSC mobilization , collectionand
transplantation , includingtheadversereactionof17.4% ( 8 / 46 ) inAPBSCmobilization , 10.9%
(
5 / 46 ) inAPBSCcollection , and15.2% ( 7 / 46 ) intransplantation.Conclusion TheMNCand
CD34
+
cellscanbegreatlyincreasedafterAPBSC mobilizationandcollection , indicatingsafty
andfeasibilityofAPBSCTformyocardialinfarction.

Key words: myocardialinfarction , coronaryarterybypassgrafting ;
off-pump , stemcell
transplantation