Comparison of liver tumor models of rabbit inoculated with VX2 tumor through liver and spleen

doi:10.3969/j.issn.10073205.2016.08.018

Abstract

Abstract: [
Abstract ] Objective Toobservewhethertherabbitlivertumormodelscanbesuccessfully
establishedandtoexplorethedifference betweenthe2 models.VX2tumorstrains were
inoculatedindifferentpartsoftherabbit ( liverandspleen ) .Imagingfeatureswereexaminedby
usingCTenhancedscananddigitalsubtractionangiography ( DSA ) .Methods Sixtyrabbitswere
dividedintotwogroupsrandomly ( 30rabbitsineachgroup ) .Ingroup A , thetumorcell
suspensioninavolumeof1mLwasinjectedintoliver.IngroupB , thesuspensionwasinjected
intospleen.TherabbitswereexaminedbyCTperfusionscanandDSAat14and15daysafter
implantation.TheCT manifestation ( thenumberandenhancementcharacteristicsofhepatic
lesions ), themanifestationofDSA ( thenumberandstainofthelesions ) andtheparametersof
CTperfusionscanwereobserved.Results ThrityrabbitsmodelsingroupAwereallsuccessfully
establishedandtheexperimentswerecompleted.Twenty-eightrabbitsmodelsingroupBwereall
successfullyestablishedandtheexperimentswerecompleted.Thereweresignificantdifferences
intheCTscan , DSAexaminationofmultiplefocalliverlesionsandnumberofringenhanced
lesionsinliverbetweengroupAandgroupB ( P <0.05 ) .Conclusion Therabbitlivertumor
modelwassuccessfullyestablishedbyVX2cellsuspensioncultureinliverandspleen.Themodel
establishedbyliverinoculated with VX2tumorstrain wasfoundtobe moreclosetohuman
primarylivercancerbyusingtheCTscanandDSA.Themodelestablishedbyspleeninoculated
withVX2tumorstrainwasfoundtobemoreclosetohumanprimarylivermetastasisbyusing theCTscanandDSA.

Key words: liverneoplasms , angiography , animals, laboratory

SHI Bo,PING Yong, WANG Yongzhong, LI Zhigang*. Comparison of liver tumor models of rabbit inoculated with VX2 tumor through liver and spleen[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2016.08.018.

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Comparison of liver tumor models of rabbit inoculated with VX2 tumor through liver and spleen

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