Abstract: ［Abstract］ Objective〖HTSS〗〓To study the effect and the significance of benzalkonium（BZK） chloride on blood endothelin(ET) and nitric oxide(NO) concentrations of rabbits surgical model of vascular anastomosis of BZK chloride on vascular anastomosis in rabbits model of dynamic blood ET and NO and the significance of the effect of concentration, and to elucidate the relationship between different concentration of BZK chloride solution and the degree of stimulation of vascular wall.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The 48 New Zealand white bigearrabbits were divided randomly into control group(saline group), BZK chloride solution 0.01%, 0.05%, 0.1% group. An experimental animal model of vascular anastomosis was established. The changes of blood ET and NO concentration in the experimental animals were detected and analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓After the operation, the levels of ET in each group were decreased gradually, but the difference was not statistically significant(P＞005). At the same time, the level of ET was increased gradually in each group. By 6 h after operation, it was higher in group C and D than in group A and B. By 12 h after operation, it was higher in group D than in group A and group B. By 24 h after operation, it was higher in group D than in group A, B, C, the difference was statistically significant(P＜005). At different time points after operation, the level of NO was increased gradually. There was a significant difference in group A, group B and group C(P＜005), but there was no significant difference between group D(P＞005). At the same time point, the NO level of each group was decreased gradually. By 6 h and 12 h after operation, it was lower in group C and D than group A and B. By 24 h after operation, it was lower in group B, C and D than group A, and the difference was statistically significant(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The consistency of BZK chloride for vascular anastomosis surgery should be 0.01%, which can reduce the stimulation to vascular wall, reduce vascular spasm after the surgery, help the wound end unobstructed, and prevent blood vessel from danger.

Key words: vascular anastomosis; , model; , animal; , benzalkonium