Abstract: ［Abstract］〓Objective〖HTSS〗〓To explore the clinical diagnosis value of colloidal gold method for simultaneously detection of MPIgM and MPIgG in children with mycoplasma pneumoniae(MP) infection. 
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The 344 suspected patients with MP infection were studied. MP antibody titer was detected by pellet agglutination assay, and MPIgM and MPIgG were simultaneously detected by colloidal gold method. Consistency of the results tested by the two methods was compared.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓When the antibody titers of 1〖DK〗∶160 were considered as positive by pellet agglutination assay, 233(67.7%) cases were negative for MP and 111(32.3%) cases were positive for MP. The results tested by diagnostic kit for IgM antibody to MP and diagnostic kit for IgG antibody to MP were roughly consistent with diagnostic kit for IgM and IgG antibody to MP. Using the results of clinical diagnosis as the gold standard, the sensitivity and negative predictive value of pellet agglutination assay were 100.0% when the critical value at the antibody titers of 1〖DK〗∶40, which was accordant with those of pellet agglutination assay when the critical value at the antibody titers of 1〖DK〗∶160. However, the specific, positive predictive value and accuracy of pellet agglutination assay when the critical value at the antibody titers of 1〖DK〗∶40 were significantly lower than those of pellet agglutination assay when the critical value at the antibody titers of 1〖DK〗∶160. The sensitivity, specific, positive predictive value, negative predictive value and accuracy difference between MPIgM, MPIgG and pellet agglutination assay when the critical value at the antibody titers of 1〖DK〗∶160 were basically identical.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Colloidal gold method can simultaneously detect MPIgM and MPIgG of clinical suspected with MP infection, thus improving disease diagnosis rate, shortening the course of the disease, avoiding crossing infection and reducing the financial burden on patients and society.

Key words: pneumonia, mycoplasma, immunoglobulin M, immunoglobulin G