Abstract: ［Abstract］ Objective〖HTSS〗〓To develop a ultra high performance liquid chromatography tandem mass spectrometry(UPLCESIMS/MS) method for the simultaneous determination of 12 compounds(βecdysterone, ajugasterone C, hederasaponin C, cirenshenoside S, pulsatilloside C, C47H76O17, anemoside B4, hederasaponin B, anemoside A3, αhederin, 23hydroxybetulinic acid and hederagenin) in Baipuhuang capsules.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The chromatographic separation was performed on a Phenomenex KinetexC18 column (100×3 mm, 2.6 mm) and the column temperature was set at 40 ℃. The mobile phase, 0.1%(v/v) formic acid (A) and methanol consisted of 0.1% formic acid (B), were run at a flow rate of 0.4 mL/min and the analytes were eluted with a gradient system. Multiplereaction monitoring scanning was employed for determination with positive and negative modes. The conditions of MS/MS detector were set as follows: source temperature 150 ℃, capillary voltage 2.0 kV, and desolvation temperature 350 ℃, desolvation and cone gas at a flow rate of 800 and 150 L/h, respectively. Atomization gas pressure 7.0 Bar and the interface heater was turned on.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The complete separation was obtained within 14 min for the 12 compounds(βecdysterone, ajugasterone C, hederasaponin C, cirenshenoside S, pulsatilloside C, C47H76O17, anemoside B4, hederasaponin B, anemoside A3, αhederin, 23hydroxybetulinic acid and hederagenin). Seven regression equations showing linear relationships between peak area and content of each compound were obtained. The recoveries(n=6) of the compounds were 97.13%-101.2% and relative standard deviation were 1.2%-4.1%, respectively.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The analytical method established in this study posseses the advantage of simple operation, rapid speed, strong specificity and high sensitivity, and can be employed to the simultaneous quantification of multiple components in Baipuhuang capsules.

Key words: Baipuhuang capsules, chromatography, high pressure liquid； quality control