Abstract: [
Abstract ] Objective ToobservetheexpressionlevelsofmiR-16inplasmaandsynovial
fluidfrompatientswithrheumatoidarthritis ( RA ), andtoexploreitsvalueinearlydifferented
diagnosisandin predictionofdiseaseseverityof RA.Methods Plasma , peripheralblood
mononuclearcell ( PBMCs ) andsynovialfluidweretakenfrompatientswithRAandosteoarthritis
( OA ) .The expression level of miR-16 was detected by stem-loop real-time fluorescent
quantitativepolymerasechainreaction ( stem-loopFQ-PCR ) withSYBRGreenⅠdye , andusing
U6snRNAasendogenouscontrol.Results Bothoftheamplicationcurvesandthedissociation
curvesofmiR-16andU6snRNA wererunningwell.Noprimerdimmerswereformed , andthe
productsofPCRwerespecific.TheexpressionlevelofmiR-16inplasmawashigherthanthosein
synovialfluidandPBMCs , respectively ( P <0.01 ) .TheexpressionlevelofmiR-16inPBMCs
washigherforRApatientsthanforhealthycontrols ( P <0.01 ), thelevelinsynovialfluidwas
higherforRApatientsthanforOApatients ( P <0.01 ) .TheexpressionlevelofmiR-16inplasma wasinverselycorrelated withtheDisease ActivityScore ( DAS28 )( P <0.05 ), butwasnot
correlatedwitherythrocytesedimentationrate ( ESR ) andC-reactiveprotein ( CRP ) .themiR-16
levelinPBMCswaspositivelycorrelatedwithDAS28 , ESRandCRP ( P <0.05 ) .ThemiR-16
levelinsynovialfluidhadnocorrelationswithDAS28 , ESRandCRP ( P >0.05 ) .Conclusion
Stem-loopFQ-PCR with SYBR Green Ⅰdye wasasensitiveandspecific methodforthe
quantitativedetectionofmiRNAs.Thereweredistinctprofilesinplasmaandsynovialfluid , and
theexpressionlevelofmiR-16insynovialfluidcanbeamarkerfordifferentialdiagnosisofRA
andOA.Theexpressionlevelofplasma miR-16canserveasaneffectivenovelindicatoron
assessmentoftheclinicaldiseaseactivity.