Journal of Hebei Medical University

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A comparative study on the effects of dexamethasone pretreatment and treatment on acute myocardial injury induced by isoproterenol in mice

  

  1. 1.The First Department of Sanatorium, Hangzhou Sanatorium of Nangjing Military Area Command, Zhejiang Province, Hangzhou 310007, China; 2.Institute of Immunology, Medicine School of Zhejiang University, Hangzhou 310058, China; 3.Department of Neurosurgery, 117 Hospital of PLA, Zhejiang Province, Hangzhou 310013, China
  • Online:2016-10-25 Published:2016-11-04

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Abstract: [
Abstract ] Objective Toinvestigatethepotentialprotectiveeffectofglucocorticoidreceptor
agonistdexamethasone onisoproterenol ( ISO ) -induced myocardialinjury and the possible
mechanism.Methods Thesixteenmicewererandomlydividedintocontrol ( CON ) group ( n =4 ),
ISOgroup ( n =4 ), dexamethasonepretreatment (
DEX-pre ) group ( n =4 ) anddexamethasone
treatment ( DEX ) group ( n =4 ) .ThemiceinCONgroupandISOgroupweregiventhesame amountof0.9% sodium chlorideand DEX-pregroup given dexamethasone ( 1.25 mg /
kg
)
injectionbyintraperitonealinjectionasapretreatmentat30 minutesbeforetreatment.CON
groupwasgiventhesameamountof0.9%sodiumchlorideinjectionbyintraperitonealinjection ;
ISOgroup , DEX-pregroupandDEXgroupreceived5mg
/ kgisoproterenolhydrochloride , daily
intraperitonealinjection of1time , for3 consecutive days.Atthesecond and third day ,
dexamethasone ( 1.25 mg /
kg
) wasgivenafterISOinjectedfor30 minutesin DEX group.
MyocardialtissueinjurywasassessedbyHEstaining.Flowcytometrywasadoptedtodetectthe
expressionofCD4
+
T , CD8
+
T , CD4
+
CD69
+
T , CD8
+
CD69
+
Tcellsinspleen.Results ①
ComparedwithISOgroup , thedegreeofmyocardialinjuryweregreatlyimproved (
P <0.05 );
thedegreeofmyocardialinjurywasn'tsignificantlydifferenceinDEXgroup ( P >0.05 ) .②The
expressionofTNF-αinseruminDEXgroupwassignificantlydifferencetotheothergroup ( P <
0.05 ) .③Theexpression0fCD4
+ T ,
CD4
+ CD69 + T ,
CD8
+ CD69 + TcellsinDEX-pregroupwas
significantlydifferencetotheothergroup ( P <0.05 ) .TheexpressionofCD8
+ TcellsinDEX-pre
groupwassignificantlyreducedtotheothergroup ( P <0.05 ) .TheexpressionofCD8
+ Tcellsin
DEXgroupwassignificantlyreducedtothecontrolgroupandISOgroup ( P <0.05 ) .Conclusion
DEX pretreatment has protective effect against ISO-induced myocardial injury. But
dexamethasonetreatmentdon'thaveprotectiveeffectafterinjury.Themechanism mightbethe
relatedwiththechangesofimmunefunctionandchangesofinflammatorycytokines.Theexact
mechanismremainstobefurtherstudied

Key words: myocardialischemia , dexamethasone , isoproterenol