Journal of Hebei Medical University

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Effects of specific CTL which are activated by DC modified with IL27 gene and esophageal tumor lysate on esophageal carcinoma cell mitochondria membrane potential and cell apoptosis

  

  1. 1.Department of  Chest Surgery， the Hospital of Longyao County, Hebei Province Longyao 055350, China;
    2.Department of Pathology, Shexian Hospital of Hebei Province, Shexian 056400, China;
    3.Department of Surgery, General Hospital of Fengfeng to Jizhong Energy Group,
    Handan 056201, China; 4.Department of Dermatology, General Hospital of
    Fengfeng to Jizhong Energy Group,Hebei Province Handan 056201, China;
    5.Department of Thoracic Surgery, Fourth Hospital of Hebei
    Medical University，Shijiazhuang 050011, China
  • Online:2016-12-25 Published:2016-12-29

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Abstract: [ Abstract ] Objective Toexploretheeffectofspecificcytotoxiclymphocyte (
CTL ) whichare
activatedbydendriticcell ( DC ) modified withIL-27 geneandesophagealtumorlysateon
esophagealcarcinomacellapoptosis.Methods After makingthe nude mice models with
esophagealcancercellsuccessfully , the mice wereimmunized withspecific CTL whichare
activatedbyIL-27genemodifiedDCloadedwithesophagealtumorlysate ( DC IL-27+Ag ) .Thecell apoptosisratewasanalyzedbyAnnexinV / PIstaining.Themitochondrialmembranepotential
was measuredby Rhodamine123stainingandtheexpressionlevelofcaspase-3protein was
detectedbyflowcytometry.Results Theratioofapoptosis , membranepotentialofmitochondria
andexpressionofcaspase-3of5groupsshowedsignificantdifference ( P <0.05 ) .Theratioof
apoptosisandcaspase-3proteinexpressioninDC IL-27+Ag group , DC IL-27 groupandDC naive group
werehigherthanthoseinPBSgroup ( controlgroup ) .Themembranepotentialofmitochondriain
DC IL-27+Ag group , DC IL-27 groupandDC naive groupwashigherthanthatinPBSgroup.Theratioof
apoptosisandcaspase-3proteinexpressioninDC IL-27+Ag , group , DC IL-27 groupand , DC naive group
werehigherthanthoseinTnaivegroup , butthemembranepotentialofmitochondriainDC IL-27+Ag
group , DC IL-27 groupandDC naive groupwaslowerthanthatinTnaivegroup.Theratioofapoptosis
andcaspase-3proteinexpressioninDC IL-27+Ag group , DC IL-27 group werehigherthanthosein
DC naive group , butthemembranepotentialofmitochondriainDC IL-27+Ag group , DC IL-27 groupwas
lowerthanthatin DC naive group.Theratioofapoptosisandcaspase-3proteinexpressionin
DC IL-27+Ag group , were higherthanthosein DC IL-27 group , butthe membrane potentialof
mitochondriainDC IL-27+Ag groupwaslowerthanthatinDC IL-27 group.Conclusion SpecificCTL ,
activatedbyDCmodifiedwithIL-27geneandesophagealtumorlysate , efficientlyplaycytotoxic
effectontumor-bearing miceinvivothroughreducingthemitochondrialmembranepotential ,
initiatingth

Key words: sophagealcarcinoma , dendriticcells , interleukin27 , cellapoptosis