Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the correlation between the change of  adenosine triphosphate（ATP）, 2,3diphosphoglycerate（2,3DPG) concentration and oxidative stress level in suspended red blood cells without leukocyte during storage.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The blood of volunteers were collected to prepare suspended red blood cells without leukocyte. The suspended red blood cells were stored at 2-6 ℃. On day 7, day 14, day 28 and day 42 of storage, the concentration of reactive oxygen species（ROS) in red blood cells was detected by fluorescence microplate reader, the concentration of hydrogen peroxide（H2O2) outside cell was detected with spectrophotometric method, the concentration of ATP was detected with chemical chromatometry, the 2,3DPG was detected with Uv test method. Correlation analysis between the ROS, H2O2, ATP, 2,3DPG and storage time were carried out, respectively. Correlation analysis between ATP, 2,3DPG and ROS, H2O2 were carried out, respectively.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓With the extension of storage time, the concentration of ROS and H2O2 were significantly increased， the ROS and H2O2 content on day 42 of storage were significantly higher than that of day 7, day 14 and day 28. There were positive correlation between ROS and storage time, between H2O2 and storage time. But with the extension of storage time, the concentration of ATP and 2,3DPG were significantly decreased. The ATP and 2,3DPG content on day 42 of storage were significantly lower than that of day 7, day 14 and day 28. There were negative  correlation between ATP and storage time, between 2,3DPG and storage time. There were negative correlation between ATP and ROS, between ATP and H2O2, between 2,3DPG and ROS, between 2,3DPG and H2O2.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓With the extension of storage time, the concentration of ATP and 2,3DPG in suspended red blood cells without leukocyte were gradually reduced, which is closely related with the increasing of oxidative stress reaction. 

Key words: blood preservation, adenosine triphosphate, 2,3diphosphoglycerate