Abstract: ［Abstract］ Objective〖HTSS〗〓To develop an analysis method for Rhein, Emodin, Chrysophanol and Physcion in Mingmu Shangqing tablets and stibene glucoside in Jiangzhining tablets by high efficiency liquid chromatography（HPLC）.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The four constituents of Mingmu Shangqing tablets were separated on Kromasil C18 column (250 mm×4.6 mm, 5 μm) using a mobile phase consisted of methanoltetrahydrofuran1% phosphoric acid in water at the detection wavelength of 254 nm. The constituent stibene glucoside in Jiangzhining tablets was separated on Kromasil C18 column (250 mm×4.6 mm, 5 μm) using a mobile phase consisted of methanol 0.1% phosphoric acid in water at the detection wavelength of 320 nm.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Rhein, Emodin, Chrysophanol and Physcionhad had a good liner relationship within the scope of 0.500 to 4.00(r=0.999 3), 0.370 to 2.96(r=0.999 0), 1.10 to 8.80(r=0.999 0), 1.00 to 8.00(r=0.999 0) ng, respectively. And their regression equation were Y=8.60×103+9.62×104X, Y=-1.16×104+1.20×105X, Y=7.41×103+1.20×105X, Y=4.13×103+6.91×104X, respectively. The average recoveries（n=6）were 97.8%， 97.8%， 98.7%， 99.5% and with relative standard deviation（RSD）（n=6） of 1.2%， 0.89%， 1.7%， 1.7%. Stibene glucoside had a good liner relationship within the scope of 0.218 to 1.31 ng(r=0.999 0). The regression equation was Y=-6.33+3.18×103X. The average recoveries were 96.6% and with RSD of 0.65%.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The method is rapid,simple,accurate and with good reproducibility and can be used in quality control of Mingmu Shangqing tablets and Jiangzhining tablets.

Key words: Rhein, stibene glucoside, chromatography, reversephase