Abstract: ［Abstract］  Objective〖HTSS〗〓To improve the method for the determination of praeruptorin A and praeruptorin B in Peucedani Radix by high performance liquid chromatography. Then the method was used for the analysis of commercial “Qian Hu”.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Methanol was used as the extraction solvent and ultrasonic extraction time was 30 min. The extract was analyzed directly. The separation was performed on a Thermo ODS HYPERSIL(4.6×250 mm, 5 μm) column using methanol and water(68〖DK〗∶32) as mobile phase.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The methodology validation presented that praeruptorin A and praeruptorin B were in good correlation in the range of 5.00-400.00 mg/L and 0.50-40.00 mg/L with the regression equations of Y=28.478 222 4X+6.484 094（r=0.999 99） and Y=25.589 318 7X-0.328 822（r=0.999 99）, respectively. The results of the precision (RSD) and recoveries were in the range of 0.6%-2.0% and 90.7%-104.8%, respectively. Nine batches of commercial “Qian Hu” were determined by the established method.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The method is simpler, more rapid and more reproducible. It can be used for the accurate determination of praeruptorin A and praeruptorin B in Peucedani Radix.

Key words: Peucedani Radix, praeruptorin A, praeruptorin B, high performance liquid chromatography