Abstract: ［Abstract］ Objective〖HTSS〗〓To assess the TanshinoneⅡA antiproliferative effects on K562 cells in vitro, and investigate the underlying mechanisms.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓CCK8 method was used to detect the proliferation of the and AnnexinV/PI flow cytometry was used to detect the apoptosis rate. The expression of PKM2 mRNA was detected by RTqPCR. Western blot was used to detect the PKM2, PCNA and CleavedCaspase3 protein expression. The transfection efficiency of PKM2 overexpression vector was detected by Western blot and RTqPCR.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓TanshinoneⅡA significantly inhibited the proliferation of K562 cells in a dosedependent manner. TanshinoneⅡA also increased K562 cells apoptosis.  Tanshinone ⅡA downregulated PKM2 mRNA and protein expression in K562 cells. Tanshinone IIA also downregulated PCNA protein expression and upregulated CleavedCaspase3 expression.  Overexpresssion PKM2 reversed apoptosis induced by TanⅡA in K562 cells.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Tanshinone Ⅱ A can inhibit the proliferation and induce apoptosis of K562 cells. It may be through downregulating the expression of PKM2 to influence the aerobic glycolysis in K562 cells to play its antileukemia role.

Key words: leukemia, myeloid, Tanshinone, pyruvate kinase