Abstract: ［Abstract］ Objective〖HTSS〗〓To observe the expression change of glutathione peroxidase1(GPX1) in rats of hepatic ischemiareperfusion injury(HIRI) model and investigate the role of GPX1 in scavenging peroxide and reducing peroxidative injury induced by ischemia and reperfusion.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The HIRI model of rats was established by blocking the hepatic pedicle of the right and middle lobes for 30 mins. The blood and liver were taken after 6 hours of reperfusion. The activity of serum ALT was detected with rate method, the changes of liver morphology and structure in rats was observed by HE staining, the malondialdehyde（MDA） and hydrogen peroxide(H2O2 ) content in liver were determined by Thiobarbituric acid colorimetric method，and Molybdate colorimetric method respectively. The GPX1 mRNA and protein expression level were 〖JP2〗evaluated by RTPCR and Western blotting respectively, the correlation between the GPX1〖JP〗 expression level and the MDA content, between the GPX1 expression level and the H2O2 content were analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the rats of control group, the morphological structure of liver of HIRI group was significantly damaged, the serum ALT activity, the content of MDA and H2O2 in the liver of HIRI model were significantly increased, the mRNA and protein expression level of GPX1 in liver were also significantly enhanced, There were positive  correlation between GPX1 mRNA and MDA, 〖JP2〗between GPX1 pretein and MDA, between GPX1 mRNA and H2O2, between GPX1〖JP〗 pretein and H2O2.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Ischemia and reperfusion causes liver tissue to be in oxidative stress and been subjected to peroxidative damage. Antioxidant enzymes GPX1 may play an antioxidant stress role by scavenging peroxides during the process, and reduce hepatic peroxidative injury induced by ischemiareperfusion.

Key words: reperfusion injury, glutathione peroxidase, hydrogen peroxide