Abstract: ［Abstract］ Objective〖HTSS〗To investigate the effects of different total RNA extraction methods and different reverse transcription primers on circular RAN amplification Hep2 cell and provide a reliable experimental method for the identification and amplification of circular RNA.
 〖WTHZ〗Methods〖HTSS〗Total RNA was extracted from prostate cancer cell line Hep2 cells by using Trizol method and miRNeasy Mini kit protocol. Total RNA was quantified by NanoDorp 2000. Random primer and oligoDT primer were used to reverse transcription of total RNA. Real-time quantitative polymerase chain reaction(RT-qPCR) was used to detect the expression of cicHIPK3 and circFLNA.
 〖WTHZ〗Results〖HTSS〗The concentration of RNA extracted by Trizol method was higher than that of RNA extracted by miRNeasy Mini Kit （P＜0.01）. The purity of RNA extracted by miReasy Mini Kit was higher than that of RNA extracted by Trizol method. The reverse transcription could not amplify the above two circular RNAs, but the universal primers were used for transcription, and both circular RNAs could be amplified; Real-time quantitative polymerase chain result showed that the relative expression of circHIPK3 and circFLNA was higher in miRNeasy Mini method group than in Trizol method(P＜0.01).
 〖WTHZ〗Conclusion〖HTSS〗The purity of RNA extracted by miRNeasy Mini Kit is higher, which is more conducive to the amplification of circular RNA.

Key words: ribonucleotides, polymerase chain reaction, nucleic acid amplification techniques