Abstract: Objective To investigate the distribution of ABO，RhD blood type in Fengfeng mining area of Handan City, Hebei Province, to analyze the results of irregular antibody screening, and to explore the clinical significance of ABO、 Rh blood type investigation and establishment of Rh phenotype bank in safe blood transfusion.
Methods The results of ABO blood type and RhD blood type of 8 000 inpatients in our hospital were collected. The irregular antibody was detected in 5 000 patients who needed blood transfusion. The positive samples were further identified with antibody specificity. Three thousand bags of RhD positive filter red blood cell suspension were randomly selected to detect Rh phenotype, and the Rh phenotype database of blood donors was established by computer to explore its significance and value in blood transfusion.
Results The distribution of ABO blood type in 8 000 patients was B blood(32.02%), O blood(28.98%), A blood(27.01%), AB blood(11.99%). There was significant difference in ABO blood type distribution among different age groups(P＜0.05), but there was no significant difference in ABO blood type distribution between different sex groups(P＞0.05). RHD was negative in 48 of 8 000 patients(0.60%). There was no significant difference in Rh blood type distribution between male and female(P＞0.05). Among the 5 000 blood samples of patients who needed blood transfusion, 30(0.60%) were positive for irregular antibody, 19(63.33%) were positive for Rh blood type system antibody, and the detection rate of anti-E was the highest. The detection rate of irregular antibody in female was significantly higher than that in male(P＜0.05). There were 8 Rh blood type phenotypes in 3 000 RhD positive blood donors, of which CCDee was the most frequently detected, followed by CcDEe，DcDEE and ccDee were less frequently detected.
Conclusion The distribution of ABO blood type is related to region and age. In addition to irregular antibody screening before blood transfusion, the antibody specificity should be further tested. The establishment of Rh phenotype database of blood donors is conducive to prevent hemolytic blood transfusion reaction caused by Rh phenotype mismatch, which is of great significance for clinical safe blood transfusion.

Key words: ABO blood-group system, Rh-Hr blood-group system, irregular antibody Rh phenotype database