Journal of Hebei Medical University ›› 2020, Vol. 41 ›› Issue (11): 1296-1300.doi: 10.3969/j.issn.1007-3205.2020.11.012
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Abstract: Objective To investigate the regulation effect of autophagosomes and apoptotic bodies on bronchopulmonary dysplasia(BPD) in neonatal mice. Methods Thirty-six neonatal SD mice were randomly and equally divided into three groups for different treatments, control group, model group and intervention group. On the 22nd day, the general situation of mice in each group was observed, the pathological changes of lung tissue were observed by HE staining, and the ultrastructure of type Ⅱ alveolar epithelial cells in each group was observed by transmission electron microscopy. The expression levels of autophagy related proteins [P62, microtubule-related protein light chain 3B(LC3B)] and apoptotic related proteins [Bcl-2-related X protein(Bax), caspase-3] in lung tissues were detected by Western blot. Results Compared with the model group, the mental state, hair growth, diet, breathing and body weight of the mice in the intervention group were significantly improved, with no mice died. Moreover, the pathological changes of lung tissue in the intervention group were also significantly improved. The number of autophagosomes and apoptotic bodies in the cytoplasm of type Ⅱ alveolar epithelial cells in the intervention group was significantly lower than that of the model group. The levels of LC3B, P62, Bax and caspase-3 in the lung tissue among three groups were the highest in the intervention group, followed by model group and control group, with statistic difference(P<0.05). Conclusion After excessive autophagy activation of type Ⅱ alveolar epithelial cells in newborn mice, autophagosomes are increased, which promotes a large number of apoptosis of type Ⅱ alveolar epithelial cells and increases the apoptotic corpuscles, thus leading to the occurrence of bronchopulmonary dysplasia, so it can provide theoretical support for the prevention and treatment of BPD.
Key words: bronchopulmonary dysplasia, apoptosomes, autophagy
ZHENG Yu, LI Lan, HU Hui-ping, LI Zheng-tao, LIU Wen-chun, PENG Yi-jie. Regulation of autophagosomes and apoptotic bodies on bronchopulmonary dysplasia in neonatal mice[J]. Journal of Hebei Medical University, 2020, 41(11): 1296-1300.
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URL: https://xuebao.hebmu.edu.cn/EN/10.3969/j.issn.1007-3205.2020.11.012
https://xuebao.hebmu.edu.cn/EN/Y2020/V41/I11/1296