Journal of Hebei Medical University ›› 2021, Vol. 42 ›› Issue (9): 1003-1007,1015.doi: 10.3969/j.issn.1007-3205.2021.09.003

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MiR-18B-5p regulates BTG3 and downstream signaling pathways to promote the development of hepatocellular carcinoma

  

  1. .Department of Radiotherapy, the Fourth Hospital of Hebei Medical University, Shijiazhuang 
    050011, China； 2.Department of Endocrinology, the Fourth Hospital of Hebei Medical 
    University, Shijiazhuang 050011, China； 3.Medical Record Room, the Third 
    Hospital of Hebei Medical University，Shijiazhuang 050051, China； 
    4.Department of Laboratory Medicine, the Fourth Hospital of 
    Hebei Medical University， Shijiazhuang 050011, China
  • Online:2021-09-25 Published:2021-09-28

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Abstract: Objective To explore the role and the mechanism of miR-18B-5p in hepatocellular carcinoma(HCC). 
Methods The expression levels of miR-18B-5p were measured in serum of patients with HCC, and its clinical relevance was analyzed. The miR-18B-5p level was down-regulated and the role of miR-18B-5p in the proliferation, migration and invasion in HCC cell lines was examined. In addition, its mechanism was detected by qRT-PCR and luciferase reporters. 
Results The survival rate of patients in low miR-18B-5p expression group was higher than that in high miR-18B-5p expression group(P＜0.05). Multivariate analysis showed that the expression level of miR-18B-5p(RR: 2.064, 95%CI: 1.522-2.800) and age(RR: 1.762, 95%CI: 1.347-2.305) were the influencing factors of survival. The level of miR-18B-5p expressed by HepG2 cells was higher than that in BEL-7402 group, MHCC97 group and Hep3B group(P＜0.05). The expression of Si-miR-18B-5p and cell proliferation of Si-miR-18B-5p cell line were lower than those of HepG2 cell line(P＜0.05). In Si-miR-18B-5p cell line, miR-18B-5p was down-regulated, and the ability of cell migration and invasion was lower than that of HepG2 cell line(P＜0.05). The mRNA expression of BTG3 in Si-miR-18B-5p cell line was higher than that of HepG2 cell line(P＜0.05). The relative fluorescein activity of BTG3 WT was lower than that of BTG3 Mut(P＜0.05). 
Conclusion MiR-18B-5p may be a new therapeutic target and prognostic biomarker for HCC.


Key words: carcinoma, hepatocellular, biomarkers, pharmacological, MiR-18B-5p