Journal of Hebei Medical University ›› 2022, Vol. 43 ›› Issue (8): 872-876.doi: 10.3969/j.issn.1007-3205.2022.08.002

Previous Articles     Next Articles

Study on the protective effect of early enteral nutrition on intestinal mucosal structure and microecology of young rats with biliary atresia

  

  1. Department of General Surgery, Shenzhen Children′s Hospital, Guangdong Province, Shenzhen 518026, China

  • Online:2022-08-05 Published:2022-09-13

Abstract: Objective To investigate the protective effect of early enteral nutrition on intestinal mucosal function and microecology in young rats with biliary atresia(BA). 
Methods In total, 40 successfully established BA rat models were divided into the sham operation group, parenteral nutrition(PN) group, enteral nutrition(EN) group and antibiotic group by random number method, with 10 mice in each group. Another 10 young rats were selected as sham operation group. All groups had free access to water, the sham operation group was fed with common diet by gavage, and the antibiotic group was given common diet combined with ampicillin by gavage; The PN group was treated with total parenteral nutrition, and the EN group was treated with total enteral nutrition. The weight of the rats in each group was weighed before and after modeling. At 10 d after modeling, young rats were sacrificed, and blood was collected to detect total protein(TP) and albumin(ALB) in serum. Small intestinal tissue was stained with hematoxylin-eosin staining, and the mesenteric lymph nodes were cultured for bacteria. Body weight, nutritional status, small intestinal mucosa morphology(thickness, depth, height) and mesenteric lymph node bacterial culture were compared among the groups. 
Results After the success of modeling, the weight of young rats in each group decreased first and then increased. Compared with the sham operation group, the weight of antibiotic group, EN group and PN group increased slowly after weight loss. There were significant differences in the interaction between the four groups, time points and time points between groups(P<0.05). At 10 d after treatment, compared with the sham operation group, the levels of TP and ALB in the antibiotic group and PN group decreased, and compared with the antibiotic group and PN group, the levels of TP and ALB in the EN group increased(P<0.05). Compared with the sham operation group, the mucosal thickness, the depth of glandular recess and the height of villi in the antibiotic group and PN group decreased, which, however, increased in the EN group compared with the antibiotic group and PN group(P<0.05). Mesangial lymph node bacterial culture showed that compared with the sham operation group, the number of Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa and Klebsiella pneumoniae in the antibiotic group and PN group increased, and the number of the above bacteria in the EN group decreased compared with the antibiotic group and PN group(P<0.05). 
Conclusion Early enteral nutrition therapy can increase the body weight of BA young rats, improve nutritional status and intestinal structure, promote intestinal mucosal repair and stabilize microecological balance.


Key words: biliary atresia, enteral nutrition, intestinal mucosa