Journal of Hebei Medical University ›› 2022, Vol. 43 ›› Issue (12): 1390-1396,1438.doi: 10.3969/j.issn.1007-3205.2022.12.005
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Abstract: Objective To analyze the expression and clinical significance of VNN1 gene in acute myeloid leukemia(AML) through online database mining. Methods In this study, we analyzed the expression level of VNN1 gene in AML patients and normal control groups based on the cancer genome atlas (TCGA), the genotype-tissue expression (GTEx), and gene expression omnibus (GEO) databases. The correlation of VNN1 gene expression level with clinical characteristics and overall survival was analyzed with TCGA and GEO datasets by downloading the data information of AML patients. The STRING database was used to analyze VNN1 protein-protein interaction (PPI)network, and GO gene enrichment and KEGG pathway enrichment were analyzed. The correlation between VNN1 and immune cell infiltration was evaluated by single-sample gene set enrichment analysis (ssGSEA). Results Compared with healthy controls (0.025±0.053), VNN1 was significantly highly expressed in AML (3.306±1.991) (P<0.001), and its expression level was closely associated with AML patients′ cytogenetic risk, FLT3 mutation status, and NPM1 mutation (P<0.05). The receiver operating characteristic (ROC) curve showed that the expression level of VNN1 could accurately distinguish the normal population and AML patients (AUC=0.969). The K-M curve showed that the median survival time of AML patients with low VNN1 expression was 27.4 months, which, however,was only 10.1 months in the high expression group, suggesting significant differences (P<0.001). Univariate and multivariate COX regression analyses showed that high expression of VNN1 was an independent risk factor for the overall prognosis of AML (HR=1.998, 1.763) (P<0.05). STRING database showed that the proteins interacting with VNN1 included PANK1, PANK3, PANK2, VNN2, VNN3, SERPIN2, TAAR2, TAAR5, TAAR1 and STX7. The enrichment analysis of GO function and KEGG pathway showed that VNN1 mainly participated in biological processes including SNARE interactions in vesicular transport, pantothenate and CoA biosynthesis, complement and coagulation cascades, and platelet activation. Conclusion Through the analysis of online databases, NN1 is significantly highly expressed in AML and negatively correlated with the overall survival of patients, which provides a theoretical basis for further in-depth study of the pathogenesis and targeted treatment of AML.
Key words: acute myeloid leukemia, biomarker, immune cell infiltration
WU Ba-lu, LIANG Yu-xing, ZHOU Fu-ling. The expression and clinical significance of VNN1 in acute myeloid leukemia [J]. Journal of Hebei Medical University, 2022, 43(12): 1390-1396,1438.
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URL: https://xuebao.hebmu.edu.cn/EN/10.3969/j.issn.1007-3205.2022.12.005
https://xuebao.hebmu.edu.cn/EN/Y2022/V43/I12/1390