Abstract: Objective  To investigate the protective effect of butorphanol combined with flurbiprofen axetil preconditioning on renal injury induced by limb ischemia-reperfusion in mice through angiotensin (1-7) ［ANG (1-7)］ and its effect on TNF superfamily member 14 ligand (LIGHT)/TNF superfamily member 14 (HVEM) in the inflammatory pathway. 
Methods  Forty-five SPF grade BABL/c mice were randomly divided into control group (n=15), model group (n=15), and experimental group (n=15). Except the control group, renal injury model induced by limb ischemia-reperfusion was established. The experimental group was pretreated with butorphanol and flurbiprofen axetil for 12 h before modeling, and the control group and model group were given the same amount of normal saline. HE staining was used to confirm the pathological level and pathological score of kidney tissues of mice in each group. Biochemical analysis was used to determine the levels of serum creatinine (Scr) and urea nitrogen (BUN), and the levels of ANG (1-7) expression in peripheral blood and kidney tissues. The mRNA and protein expression levels of LIGHT and HVEM in kidney tissues were detected by qPCR and Western blot. 
Results  Compared with the control group, the renal tissue injury, pathological score, and the expression of Scr and BUN decreased, the expression level of ANG (1-7) decreased, and LIGHT and HVEM increased in the model group (P＜0.01). Compared with the model group, the renal tissue injury, pathological score, and the expression of Scr and BUN decreased, the expression level of ANG (1-7) increased, and LIGHT and HVEM decreased (P＜0.01). 
Conclusion  Butorphanol combined with flurbiprofen axetil preconditioning has a protective effect on renal injury induced by limb ischemia-reperfusion in mice, and its mechanism may be related to the intervention of ANG (1-7) on the expression of LIGHT/HVEM signal pathway proteins. 

Key words: reperfusion injury, renal injury, butorphanol, flurbiprofen axetil