Abstract: Objective To compare three broth dilution methods in drug sensitivity detection for detecting the sensitivity of Candida to fluconazole. 
Methods A total of 84 clinical isolates of Candida were collected in the Second Hospital of Hebei Medical University, and the sensitivity of Candida to fluconazole was detected by classical broth microdilution method, ATB FUNGUS 3 and Sensititre Yeast One method. 
Results Among the 84 strains of Candida, there were 26 isolates of Candida albicans, 34 ioslates of Candida tropicalis, 22 isolates of Candida glabrata and 2 isolates of Candida parapsilosi. The classical broth microdilution method was used as a reference method, the sensitivity of ATB FUNGUS method was 87.5%, with a specificity of 100.0%. The essential agreement was 95.2%, and the categorical agreement was 92.9%; The false resistance rate was 7.1%, while there was no false sensitivity rate. The sensitivity and specificity of Sensititre Yeast One method were 100.0% and 94.4% respectively. Both the essential agreement and the categorical agreement were 97.6%, and the false sensitivity rate and the false resistance rate were 0. 
Conclusion Sensititre Yeast One method to detect the effect of fluconazole on Candida activity is superior to that of ATB FUNGUS 3. 

Key words: candida, fluconazole, broth microdilution method