Journal of Hebei Medical University ›› 2024, Vol. 45 ›› Issue (11): 1295-1300.doi: 10.3969/j.issn.1007-3205.2024.11.010

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Experimental study on the establishment of a rat depression model by intermittent sleep deprivation combined with chronic unpredictable mild stress

  

  1. 1. Department of Prosthodontics, Stomatology Hospital of Hebei Medical University/Hebei Key 
    Laboratory of Stomatology Hebei Clinical Medical Research Centre for Oral Diseases, Shijiazhuang 
    050017, China; 2.Stomatology Class of 2020, Stomatology Hospital of Hebei Medical University, 
    Shijiazhuang 050017, China
  • Online:2024-11-25 Published:2024-11-26

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Abstract:

Objective To establish a rat model of insomnia caused by depression by using intermittent chronic sleep deprivation combined with chronic unpredictable mild stress (CUMS), and to compare the effect of CUMS with that of traditional CUMS alone, in order to explore a better modelling method for depression. 
Methods Thirty-six Sprague-Dawley rats aged 6-8 weeks were selected and randomly divided into three groups after 1 week of adaptive feeding： Group A, blank control group; Group B, CUMS group; and Group C, intermittent sleep deprivation + CUMS group, with 12 rats in each group. Each group was treated according to different depression modelling procedures, and the duration of modelling was 15 d. Behavioural tests were performed at the end of the modelling, and the concentrations of 5-hydroxytryptamine (5-HT), dopamine (DA) and norepinephrine (NE) in the hypothalamus of rats were detected by ELISA method. 
Results The intermittent sleep deprivation + CUMS group had a smaller body weight growth rate than the blank control and CUMS groups at 7 d and 14 d, a smaller sugar water preference rate than the blank control and CUMS groups, a longer immobility time than the blank control and CUMS groups, and a smaller number of traversing frames and standing than the blank control and CUMS groups (P＜0.05). In the CUMS group and the intermittent sleep deprivation + CUMS group, the hypothalamic 5-HT concentration of rats was lower than that of the blank control group, the DA concentration of rats in the intermittent sleep deprivation +CUMS group was lower than that of the blank control group, and the NE concentration of rats in the intermittent sleep deprivation+CUMS group was lower than that of the blank control group and the CUMS group (P＜0.05). 
Conclusion The use of 15-d CUMS combined with 20-h sleep deprivation at 1-d intervals can rapidly establish a stable depression model in SD rats, which has a more obvious effect on behavioural performance than CUMS alone, and has a significant advantage in terms of modelling duration. 

Key words: depression, sleep deprivation, models, animal