褪黑素对急性百草枯中毒大鼠肾损伤保护作用的研究

doi:10.3969/j.issn.10073205.2017.06.004

摘要/Abstract

摘要： ［摘要］目的观察百草枯中毒大鼠血浆尿素氮(blood urea nitrogen,BUN)、肌酐(creatinine,Cr)含量的变化，肾组织转化生长因子β1(transforming growth factorβ1,TGFβ1)的表达及褪黑素对其的影响，探讨百草枯中毒所致肾损伤的机制及褪黑素对百草枯中毒大鼠肾损伤的保护作用。方法将健康成年SD大鼠90只随机分对照组、染毒组及褪黑素组，每组30只。染毒组和褪黑素组一次性给予50 mg/kg百草枯灌胃染毒后，褪黑素组立即给予褪黑素10 mg/kg腹腔注射、1次/d，染毒组每日给予等量生理盐水腹腔注射，分别于染毒后第1、3、5、7、14天各取6只大鼠, 心脏取血，观察比较血浆BUN、Cr含量的变化，取肾组织标本行免疫组织化学法检测大鼠肾组织TGFβ1的表达。结果染毒组大鼠BUN在各时点均明显高于对照组,褪黑素组BUN在各时点均较染毒组降低，但仍高于对照组（P＜005）;染毒组和褪黑素组BUN第1~5天持续升高，第7天开始逐渐下降，差异均有统计学意义（P＜005）; 染毒组Cr在各时点均高于对照组，褪黑素组除第1天外，第3～14天均低于染毒组，但仍高于对照组(P＜005),对照组各时点之间差异无统计学意义(P＞005),染毒组和褪黑素组在第1~5天Cr持续升高，到第7天开始逐渐降低，差异均有统计学意义（P＜005）;染毒组TGFβ1水平在各时点均高于对照组，褪黑素组除了第3天外，TGFβ1均低于染毒组，但仍高于对照组（P＜005）。结论TGFβ1参与了百草枯中毒肾损伤的发病过程；褪黑素对百草枯中毒肾损伤有保护作用，其可能是通过抑制TGFβ1起作用。

关键词: 百草枯, 中毒, 褪黑素

Abstract: ［Abstract］ ObjectiveTo observe the changes of serum urea nitrogen(BUN), creatinine(Cr) in paraquat poisoning rats， and to study the mechanism of paraquatinduced renal injury in rats， the expression changes of transforming growth factorβ1（TGFβ1） to assess the protective effect of melatonin in paraquat poisoning. MethodsNinety adult healthy SpragueDawley(SD) rats were divided into three groups randomly, 30 rats in each group. Control group and poisoned group were treated intragastrically with 1mL of paraquat(50 mg/kg) diluted with normal saline. Control group were treated with the same dose of normal saline as poisoned group and melatonin group. melatonin group were given 1 mL of melatonin at a dose of 10 mg/kg diluted with normal saline(once daily, intraperitoneally). Control and poisoned group were treated with the same dose of normal saline(once daily, intraperitoneally) as melatonin group. Six rats in each group were sacrificed on the 1st, 3rd, 5th, 7th and 14th day after exposure. Blood samples were taken from the heart to observe the changes of plasma BUN and Cr. The expression of TGFβ1 in rat renal tissue was detected by immunohistochemistry. ResultsThe BUN of the rats in the poisoned group was significantly higher than that in the control group at each time point， melatonin group BUN at all time points were lower than the poisoned group，but still higher than the control group. BUN in the poisoned group and melatonin group continued to increase from day 1 to day 5, and gradually decreased on the 7th day, the difference was statistically significant（P＜005）. The Cr concentration in the poisoned group was higher than that in the control group at each time point. Except for the first, 3 days to 14 days in days group were lower than that in poisoned group(P＜005), but still higher than that in control group, and there was no significant difference between control group at each time point(P＞005). The Cr in the poisoned group and the melatonin group continued to increase from day 1 to day 5, and gradually decreased on the 7th day, the difference was statistically significant（P＜005）. The levels of TGFβ1 in the poisoned group were higher than those in the control group at each time point，melatonin group was lower than that of the control group except the 3rd day, but still higher than the control group, the difference was statistically significant（P＜005）. ConclusionTGFβ1 is involved in the pathogenesis of paraquat poisoning kidney injury. melatonin has protective effect on paraquat poisoning kidney tissue, which might be mediated by TGFβ1 in the paraquat induced renal injury，but its regulation path still need a further exploration．

Key words: paraquat, poisoning, melatonin

宫玉，陈慧，孟兆华，吕宝谱，姚冬奇，田英平. 褪黑素对急性百草枯中毒大鼠肾损伤保护作用的研究[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2017.06.004.

GONG Yu， CHEN Hui， MENG Zhaohua， LYU Baopu， YAO Dongqi， TIAN Yingping. Protective effect of melatonin on renal injury induced by acute paraquat poisoning in rats[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2017.06.004.

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褪黑素对急性百草枯中毒大鼠肾损伤保护作用的研究

Protective effect of melatonin on renal injury induced by acute paraquat poisoning in rats

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