河北医科大学学报

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TTP对小鼠乳腺癌细胞系4T1凋亡的调控

  

  1. 1.河北省滦平县医院内科，河北 滦平 068250；2.河北医科大学临床学院实验中心，河北 石家庄 050031；
    3.河北医科大学基础医学院免疫学教研室，河北 石家庄 050017
  • 出版日期:2017-02-25 发布日期:2017-03-10
  • 作者简介:朱光奎(1977-)，男，河北承德人，河北省滦平县医院主治医师，医学学士，从事内科疾病诊治研究。
  • 基金资助:
    教育部留学人员科研启动资金；河北省留学人员科技活动资助项目（2014003031）

The apoptosis regulation on mice breast cancer cell line 4T1 by TTP #br#

  1. 1.Department of Internal Medicine， the Hospital of Luanping County, Hebei Province， Luanping
    068250, China；2.The Experiment Center, Clinical College of Hebei Medical University,
    Shijiazhuang 050031, China；3.Department of Immunology, the School of Basic
    Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China
  • Online:2017-02-25 Published:2017-03-10

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摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗观察TTP (trisetetraprolin) 对小鼠乳腺癌细胞系4T1凋亡的影响。
〖HTH〗方法〖HTSS〗〖KG*2〗构建mTTP真核表达质粒PCR3.1/mTTP；脂质体转染小鼠乳腺癌4T1细胞系并筛选稳定转染克隆；用终浓度0.1 μmol的细胞凋亡诱导剂星形孢菌素处理6 h，流式细胞仪检测细胞凋亡。
〖HTH〗结果〖HTSS〗〖KG*2〗经G418筛选，成功得到稳定转染mTTP的细胞株PCR3.1+mTTP和PCR3.1（阴性对照），实时定量PCR检测mTTP的表达，4T1细胞转染PCR3.1/mTTP之后，mTTP的表达明显增高。流式细胞仪检测发现，4T1、4T1 PCR3.1和4T1 PCR3.1+mTTP 3种细胞的凋亡率差异无统计学意义，细胞凋亡诱导剂作用后PCR3.1+mTTP细胞凋亡率明显增加，凋亡率为29.5%，而4T1、4T1+PCR3.1细胞的凋亡率分别为17.0%和18.0%。
〖HTH〗结论〖HTSS〗〖KG*2〗成功构建了TTP高表达的小鼠乳腺癌细胞系PCR3.1+mTTP，该细胞对凋亡诱导药物星形孢菌素的敏感性增加。

关键词: 乳腺肿瘤, 细胞凋亡, 转染

Abstract: ［Abstract］ Objective〖HTSS〗〓To establish a mouse breast cancer cell line 4T1 with high TTP (trisetetraprolin) expression and to observe the effect of TTP on breast cancer cell line 4T1 apoptosis.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The breast cancer 4T1 cell line was stably transfected with the plasmids of PCR3.1 and PCR3.1/mTTP by Lipofectamine 2000. The cell apoptosis was detected using flow cytometry technology.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Higher mTTP expression was detected in 4T1 cells that has been transfected with PCR3.1+mTTP plasmid using real time quantitative PCR. The apoptosis rate among 4T1, 4T1 PCR3.1 and 4T1 PCR3.1+mTTP was not significant difference. But after treatment the cells with Staurosporine, a apoptosis promoting medicine, for 6 hours, apoptosis rate of 4T1 PCR3.1+mTTP cell line was increased. The apoptosis rate of 4T1, 4T1 PCR3.1 and 4T1 PCR3.1+mTTP was 17%, 18% and 29.5%, respectively.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓mTTP could not enhance 4T1 cell apoptosis directly, but it could increase the sensitivity of 4T1 to apoptosis inducing medicine.

Key words: breast neoplasms, apoptosis, transfection

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