OSAHS对雄性新西兰兔泌尿生殖系统的影响

doi:10.3969/j.issn.10073205.2018.08.014

河北医科大学学报

OSAHS对雄性新西兰兔泌尿生殖系统的影响

1.河北省儿童医院泌尿外科，河北石家庄 050031；2.河北省石家庄市第三医院口腔科，河北石家庄 050011；
3.河北医科大学口腔医院正畸科，河北石家庄 050017

出版日期:2018-08-25 发布日期:2018-07-25
作者简介:张铁军（1975-），男，河北邯郸人，河北省儿童医院副主任医师，医学学士，从事泌尿外科疾病诊治研究。

Effect of OSAHS on urogenital system of the male NewZealand rabbit#br#

1.Department of Urology, Hebei Children Hospital, Shijiazhuang 050031, China; 2.Department of
Stomatology, the Third Hospital of Shijiazhuang, Hebei Province, Shijiazhuang 050011,
China; 3.Department of Orthrodontics, the School of Stomatology, Hebei Medical
University, Shijiazhuang 050017, China

Online:2018-08-25 Published:2018-07-25

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗通过建立雄性新西兰兔阻塞性睡眠呼吸暂停低通气综合征（obstructive sleep apneahypopnea syndrome，OSAHS）动物模型，了解OSAHS对生殖系统的影响，为预防OSAHS可能导致的不育提供理论依据。
〖HTH〗方法〖HTSS〗〖KG*2〗20只雄性新西兰兔随机分为OSAHS组和正常对照组，每组10只。OSAHS组实验动物注射聚丙烯酰胺水凝胶2 mL，建立OSAHS动物模型。仰卧位睡眠8周后，处死所有动物，取睾丸组织在常规光镜、电镜下观察;取左侧附睾尾组织，检测精子计数、精子活力、精子活率及精子畸形率。
〖HTH〗结果〖HTSS〗〖KG*2〗光镜和透射电镜下可见OSAHS组动物睾丸生精细胞、生精小管、生精上皮损伤。OSAHS组动物精子计数、精子活力、精子活率明显低于对照组，精子畸形率明显高于对照组，差异有统计学意义（P＜005）；血氧饱和度与附睾尾中精子畸形率呈负相关（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗OSAHS造成的缺氧可引起生殖系统损伤，降低精子质量。

关键词: 睡眠呼吸暂停, 阻塞性, 泌尿生殖系统, 兔

Abstract: ［Abstract］〓Objective〖HTSS〗〓To set up the animal model of obstructive sleep apneahypopnea syndrome(OSAHS) in male NewZealand rabbit, and to observe the effect of the testis and the spermatozoa in cauda epididymis under OSAHS，so that to provide theoretical bases for the preventation of sterility caused by OSAHS.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Twenty male NewZealand white rabbits were randomly divided into two groups and ten rabbits in each. Two groups were obstructive sleep apneahypopnea syndrome group(group OSAHS) and control group. A total of 2 mL polyacrylamide hydrogel were injected into rabbit soft palate of group OSAHS to set up OSAHS animal models. After sleep for 8 weeks by dorsal position, all the animals were killed and the testis were observed under the transmission electron microscope and the AX80 universal microscope. The left cauda epididymis was obtained to be observed the number, viability, motility rate and abnormal rate of spermatozoa. The SPSS 13.0 software was used to analyse. 〖HTH〗〖WTHZ〗Results〖HTSS〗〓Transmission electron microscope and the light microscope revealed that the spermatogenic cell, the seminiferous tubule and the spermatogenic epithelium were damaged in the group OSAHS. In OSAHS group animals, the number, viability rate and motility rate of spermatozoa in cauda epididymis were significantly decreased while the rate of teratospermia was increased(P＜0.05). The saturation of blood oxygen and the rate of teratospermia were negatively correlated by the correlation analysis.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The damage of spermatogenic cells and the decrease of the sperm quality were caused by hypoxia which was caused by OSAHS.

Key words: sleep apnea, obstructive； urogenital system, rabbit

张铁军1，刘小莹2，乔〓星3，刘春艳3，康文静3，卢海燕3*. OSAHS对雄性新西兰兔泌尿生殖系统的影响[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2018.08.014.

ZHANG Tiejun1, LIU Xiaoying2, QIAO Xing3, LIU Chunyan3, KANG Wenjing3, LU Haiyan3*. Effect of OSAHS on urogenital system of the male NewZealand rabbit#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2018.08.014.

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OSAHS对雄性新西兰兔泌尿生殖系统的影响

Effect of OSAHS on urogenital system of the male NewZealand rabbit#br#

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