Abstract: ［Abstract］ Objective〖HTSS〗〓To observe the expression change of glutathione peroxidase1(GPX1) in lung of renal ischemia reperfusion injury(RIRI) model and to explore the role of GPX1 in protecting lung against peroxide damage by scavenging peroxide.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The left renal artery of Wistar rats was clipped with nondamage vascular clamp after removing the right kidney to create RIRI model. After 24 hours of renal blood perfusion, the blood, kidney and lung were collected. The serum blood urea nitrogen(BUN) and serum creatinine(SCr) were detected with picric acid method and enzyme coupling rate method, respectively. The morphological changes of kidney was observed with HE staining , the malondialdehyde（MDA）and hydrogen peroxide(H2O2 ) content in lung tissue were determined by thiobarbituric acid colorimetric method and molybdate colorimetric method, respectively. The GPX1 mRNA and protein expression were evaluated by RTPCR and Western blotting, respectively. The correlation between MDA content, H2O2 content and GPX1 expression level was analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the control group, the morphological structure of lung tissue of RIRI model was significantly damaged, the BUN and SCr level, the MDA and H2O2 content in lung tissue of RIRI were significantly increased, mRNA and protein level of GPX1 were also significantly enhanced. There were positive correlation between MDA and GPX1 mRNA, between MDA and GPX1 pretein, between H2O2 and GPX1 mRNA, between H2O2 and GPX1 pretein.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The RIRI can make lung tissue to be in oxidative stress and be subjected to peroxidative damage. GPX1 may protect lung against peroxide damage by scavenging superoxide.

Key words: renal artery, reperfusion injury, glutathione peroxidase 1, rats