Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the role of insulin receptor substrate2(IRS2) in the pathogenesis of type 2 diabetic osteoporosis in rats.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Sixtyfour  female Wistar rats(3months old) were randomly divided into 4 groups: normal control group(NC group), normal ovariectomy group(NOVX group), type 2 diabetes mellitus group(DC group) and diabetes ovariectomy group(DOVX group). Rats in NC and NOVX groups were fed with regular diet, while rats in DC and DOVX groups were fed with high fat diet(45%). After 8 weeks of feeding, DC and DOVX groups received an intraperitoneal injection of streptozotocin. One week later, rats in the NOVX and DOVX groups underwent ovariectomy. After 12 weeks of continuous feeding, the rat model of type 2 diabetic osteoporosis was successfully established. Body weight, fasting blood glucose, fasting insulin and insulin sensitivity index were measured. Bone mineral density and IRS2 mRNA levels were measured at 4, 8 and 12 weeks after ovariectomy.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with NC group and NOVX group, the body weight of DC group and DOVX group was decreased, while the fasting blood glucose and insulin were increased. Compared with NC group and NOVX group, insulin sensitivity index of DC group and DOVX group was decreased. Compared with NC group, the bone mineral density and IRS2 gene levels of NOVX group and DOVX group were gradually decreased at 8 and 12 weeks after ovariectomy.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓IRS2 mRNA level in bone tissue decreases with the decrease of bone density during the development of type 2 diabetic osteoporosis.

Key words: diabetes mellitus, type 2, osteoporosis, rats