Abstract: Objective  To summarize the application value of preimplantation genetic testing(PGT) in assisted reproduction. 
Methods  The clinical data, laboratory data and pregnancy outcome data of couples assisted by PGT for aneuploidies(PGT-A )and PGT for structural rearrangements(PGT-SR) in the Sixth Medical Center of PLA General Hospital were retrospectively analyzed. The embryos with normal test results after blastocyst extracorporeal trophoblastic cell biopsy and genetic testing were selected for single blastocyst transfer. The general information, embryo status, embryo biopsy results and pregnancy outcome were compared between younger(＜35 years of age)and older(≥35 years of age) women. 
Results  A total of 194 cycles, 635 embryos and 101 transfer cycles were included in PGT-A. A total of 92 cycles, 399 embryos and 40 transfer cycles were included in PGT-SR. In both PGT-A and PGT-SR, the number of retrieved oocytes of ≥35 years group was less than that of ＜35 years women(P＜0.05), and there was no statistical difference in the subsequent embryonic development(P＞0.05). However, the normal embryo rate of ≥35 years group in PGT-A and PGT-SR was significantly lower than that of ＜35 years group(P＜0.05), and with the increase of age, the normal rate of PGT-A embryo biopsy decreased gradually. There were 44 live births(43.56%) in 101 transfer cycles of PGT-A, and there was no statistical difference in pregnancy outcomes between two groups(P＞0.05). There were 19 live births(47.50%) in the 40 transfer cycles of PGT-SR, and there was no statistical difference in pregnancy outcomes between two groups(P＞0.05). All the children were healthy. 
Conclusion  PGT is an effective treatment for recurrent abortion, elderly women and couples with chromosomal abnormalities. The clinical application of PGT can effectively improve the fertility status of elderly women, however, whether women after the age of 40 are suitable for PGT should be decided after weighing the pros and cons.

Key words: reproductive techniques, assisted, preimplantation genetic testing, pregnancy outcome