Journal of Hebei Medical University ›› 2023, Vol. 44 ›› Issue (10): 1133-1140.doi: 10.3969/j.issn.1007-3205.2023.10.004

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Regulatory mechanism of miRNA on chemoresistance of oral squamous cell carcinoma

  

  1. 1.Department of Stomatology, the People′s Hospital of Shijiazhuang City, Hebei Province, Shijiazhuang 
    050000, China; 2.Department of Stomatology, the Fourth Hospital of Hebei Medical University, 
    Shijiazhuang 050017, China; 3.Department of  Stomatology, Affiliated Hospital of 
    Hebei University, Baoding 071000, China

  • Online:2023-10-25 Published:2023-11-03

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Abstract: Objective The role of characteristic microRNA (miRNA) in chemoresistance and tumor biology of oral squamous cell carcinoma(OSCC). 
Methods Three miRNAs with abnormal expression (miR-27b-5p, miR-130a-3p and miR-125a-5p) were selected from three drug-resistant OSCC cell lines SCC084/R,SCC131/R and SCC9/R. The effects of those miRNAs on cytotoxicity, apoptosis and cell cycle were evaluated in drug-resistant OSCC cell lines. Subsequently, the potential targets of miRNA were selected by Target gene prediction database TargetScan. The expression of those potential targets was evaluated by Western blotting. 
Results miR-27b-5p and miR-130a-3p could enhance the sensitivity of SCC084/R, SCC131/R and SCC9/R to cisplatin, while miR-125a-5p could only improve sensitivity of SCC9/R to cisplatin (P＜0.05). Upregulation of miR-27b-5p could increase the sensitivity of SCC9/R cells to 5-fluorouracil (5-FU), while down regulation of miR-130a-3p could increase the sensitivity of SCC084/R cells to 5-FU (P＜0.05). Compared with single miRNA transfection, co-transfected miRNA showed better chemo-sensitization in drug-resistant cell lines SCC084/Rand SCC131/R (P＜0.05). Selected miRNAs were able to cause cell apoptosis, except miR-130a-3p, and both miR-125a-5p and miR-27b-5p show preference to arrest SCC084/R and SCC131/R in G1 phase. The regulation of drug resistance involved ErbB2/Her2 pathway, p38 pathway, p53 pathway and its downstream targets. 
Conclusion miRNA may be a key factor in the regulation of drug resistance in OSCC, and multiple targets are involved during the process of regulation.


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