Journal of Hebei Medical University ›› 2024, Vol. 45 ›› Issue (3): 271-277.doi: 10.3969/j.issn.1007-3205.2024.03.004

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Analysis of the effect of m6A methylation regulatory factors on the prognosis of lung adenocarcinoma based on TCGA database

  

  1. Department of Respiratory and Critical Care, the First Affiliated Hospital of Baotou Medical College, 
    Inner Mongolia University of Science and Technology, Baotou 014040， China

  • Online:2024-03-25 Published:2024-04-07

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Abstract: Objective To investigate the expression and prognosis of m6A methylation regulatory factors in lung adenocarcinoma by bioinformatics analysis. 
Methods The transcriptome data and clinical data of 516 cases of lung adenocarcinoma were downloaded from TCGA database. The expression differences of 20 m6A methylation regulatory factors in lung adenocarcinoma tissues and paracancerous tissues were compared using R software analysis, and survival analysis was performed by univariate Cox regression analysis. Consensus Cluster Plus R method was used to perform m6A cluster survival analysis. 
Results Sixteen m6A methylation regulatory factors were differentially expressed in lung adenocarcinoma, of which 5 m6A methylation regulatory factors (IGF2BP1, IGF2BP3, HNRNPC, RBMX, YTHDC2) were significantly associated with tumor stage, 7 m6A methylation regulatory factors (IGF2BP1, IGF2BP3, HNRNPC, RBMX, METTL3, YTHDF2, METTL14) were significantly associated with T stage, and 2 m6A methylation regulatory factors (IGF2BP3, YTHDC2) were significantly associated with N stage. Cox regression analysis showed that 6 m6A methylation regulatory factors (IGF2BP1, IGF2BP2, IGF2BP3, HNRNPA2B1, HNRNPC, RBM15) were independent prognostic factors of lung adenocarcinoma. 
Conclusion m6A methylation regulatory factors are associated with the progression of lung adenocarcinoma. In addition, the 6 m6A methylation regulatory factors can be used as potential biomarkers to predict the prognosis of lung adenocarcinoma patients. 


Key words: lung adenocarcinoma, m6A methylation, TCGA database