Journal of Hebei Medical University

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Expression of SphK1 and its effect on the proliferation and metastasis of bladder transitional cell carcinoma

  

  1. 1. DepartmentofUrology , theBethuneInternationalPeaceHospital , Shijiazhuang 050082 , China ;
    2. DepartmentofUrology , SouthwestHospital , theThirdMilitaryMedical
    University , Chongqing 400038 , China
  • Online:2016-10-25 Published:2016-11-04

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Abstract: [
Abstract ] Objective Toinvestigatethesphingosinekinase1 ( SphK1 ) expressioninbladder
urothelialcarcinomaandeffectsontheproliferationand metastasisofurothelialcancercells.
Methods TheexpressionofmRNAinbladderurothelialcancertissueandadjacentnormaltissue
SphK1wasdetectedbyqRT-PCR method.Phorbol12-myristate13-acetate ( PMA ) wasusedas
SphK1activator.N-dimethylD-erythrosphingsine ( DMS ) wasusedasSphK1inhibitor.0.9%
NaClreagentwasusedasblankcontrol.BladdercancercelllineBIU-87wastreatedasactive
group , controlgroupand blankcontrolgroup.Thecellproliferationsineach group were
measuredbyCCK-8method.Matrigelmethodwasusedtoobservethevascularcellsvasculogenic
mimicry ( VM ) formingabilityinthree-dimensionalculturemethod.Results Theexpressionof
SphK1mRNAinbladderurothelialcancertissuewassignificantlyhigherthanthatinadjacent
normaltissue.SphK1activatorcanpromotetheproliferationofBIU-87cellsandtopromotethe formationof VM inthethreedimensionalculturesystem constructed by Matrigel.SphK1
inhibitorscaninhibittheproliferationofBIU-87cells , andcannotformatubularVMinthree
dimensionalculture.Conclusion SphK1 plays an importantrolein the occurrence and
developmentofbladdercancer , anditsmechanism mayberelatedtopromotingtheproliferation
ofcancercellsandpromotingtheformationofVM

Key words:  bladdercancer ;
sphingosinekinase1 , cellproliferation