Abstract: 〗［Abstract］ Objective〖HTSS〗〓To study the effects of storing suspending erythrocytes after water bath on interleukin8（IL8） in plasma, and further more to confirm the effect of water bath at various times and different degrees on IL8 in plasma. Our study will provide the theoretical basis for safe and effective use of storing suspending erythrocytes.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fifty blood samples were selected randomly from blood center in HebeiProvince, with detection of all indicators qualified. Each divided into 8 groups（A, B, C, D, E, F, G, H） which were stored at 4 ℃, heated at 37 ℃ for various time(10, 20, 30, 60, 120, 180 min) and 40 ℃ for 30 min for subsequent experiments. IL8 concentration in plasma was detected by adopt double antibody sandwich enzymelinked immunosorbent assay.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Heating could significantly upregulated IL8 level, and with timedependence pattern. There was no significant difference in plasma IL8 concentration between B and C group compared with A group(P＞005). Plasma levels of IL8 in D, E, F, G, H were significantly higher than those in the A group, the difference was statistically significant(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓There was no significant effect on plasma IL8 concentration in water bath at 37 ℃ within 30 min，and then gradually increased with time extended.

Key words: blood transfusion, erythrocyte immune functions, interleukin8