Abstract: ［Abstract］ Objective〖HTSS〗〓To explore the molecular mechanisms by which Gli transcription factor inhibitor regulates epithelialmesenchymal transition（EMT） in Lung Adenocarcinoma.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The protein expression of Gli1, Gli2, pAKT, AKT, Vimentin and βcatenin were observed by Western blot assay with respective treatment of GANT61, NShh, GANT61&NShh. Transwell assay were performed to detect cell invasion ability.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓GANT61&NShh group was lower than DMSO group and NShh protein group in expression of Gli1, Gli2, pAKT, Vimentin and the number of membrane cells, and was higher than GANT61 group in the above indicators. NShh protein group was higher than DMSO group and GANT61 group. GANT61 group was lower than DMSO group,the difference was significant（P＜001）. AKT protein expression showed no significant difference among difference groups(P＞005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Upregulated expression of Gli1 and Gli2 may promote epithelial mesenchymal transition and eventually promote cell invasion and metastasis of lung adenocarcinoma A549 cells through PI3K/AKT pathway.

Key words: lung neoplasms, epithelialmesenchymal transition, neoplasm metastasis