Abstract: ［Abstract］ Objective〖HTSS〗〓To explore the effects of different ways of treating tooth surface on pit and fissure sealant.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fortyeight isolated premolars were randomly divided into 4 groups(n=12): group A(cupshaped brush sealant technique+pit and fissure sealant), group B(enameloplasty sealant technique+pit and fissure sealant), group C(50 μm alumina oxide particles air abrasion+pit and fissure sealant), group D(27 μm alumina oxide particles air abrasion+pit and fissure sealant).The infiltration depth of the sealants were observed by stereomicroscope, and then the infiltration rate of sealants was calculated. Scanning electron microscope was used to observe the marginal adaptation and interface condition between pit and fissure sealant and tooth.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The results of scanning electron microscopy and polarized light microscopy showed that the sealant in group B, group C and group D could infiltrate into the bottom of the fossa and no cavitation occurred. Osmotic pressure in group B, group C and group D was higher than that in group A, and the difference was statistically significant(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Compared with traditional cupshaped brush pit and fissure sealant, 50 μm, 27 μm aluminum oxide particle air abrasion and enameloplasty sealant technique with highspeed tapered emery needle can effectively enhance the permeability of the sealants, etching technique and lightcured sealant can achieve a good fit and seal. In order to achieve a good seal, 27 μm particles air abrasion should be used to process the tooth with deeper pits and fissures or suspicious caries cases before pit and fissure sealant.

Key words: air abrasion, dental, acid etching, dental, pit and fissure sealant