Abstract:

［Abstract］Objective〖HTSS〗In order to study occurrence and matastasis of human ovarian carcinoma, a kind of animal model was establish via orthotopic transplantation of human ovarian mucinous adenocarcinoma 3AO cell lines.
〖HTH〗〖WTHZ〗Methods〖HTSS〗Human ovarian mucinous adenocarcinoma 3AO cells(1×107)  in exponential phase of growth were inoculated in right ovary of BALB/C(nu/nu) female. The general condition, growth and metastasis of tumor were observed. 〖JP2〗Nude mice were anatomized at 3-8 weeks after transplation, inspection by microscopy and immunohistochemical〖JP〗 analysis were applied to both ovaries and organs.
〖HTH〗〖WTHZ〗Results〖HTSS〗The success rate of orthotopic transplanted tumor model was 86.67%. Three weeks after transplantation, the right ovary became large, it grew into mass and metastasized to left ovary, intestines, liver, spleen, lung and lymph nodes, 1-3 mL ascites appeared colorless. The pathology features of transplated tumors and matastatic tumors were consistence with human ovarian mucinous adenocarcinoma by light microscopy. The expression of human carbohydrate antigen 125 were positive or strong positive. The stain areas were located in cytoplasm.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The orthotopically transplanted nude model by  human ovarian mucinous adenocarcinoma 3AO cells is established successfully. It is similar to human ovarian carcinoma in biological mode, and can be a good in vivo modle in research of human carcinoma.