Abstract: ［Abstract］ Objective〖HTSS〗〓To establish vascular calcification mouse model derived from chronic kidney disease（CKD）.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Murine CKD was induced by oral dosing with adenine. 1,25(OH)2D3 and highphosphate diet were used to accelerate vascular calcification. Serum urea nitrogen, serum creatinine, uric acid, calcium and phosphate were measured. HE staining and Von kossa staining were used to examine the morphological changes in kidney and aorta, respectively.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓After adenine dosing combined with 1,25(OH)2D3 and highphosphate diet, significant aortic calcification was detected in about 17% animals at 6 weeks, significantly increased serum urea nitrogen, creatinine, uric acid， serum calcium and phosphate were observed  and all model animals showed significant arterial wall thickening and increased medial elastic fibers.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓These data suggest that mice dosed orally with adenine in combination with 1,25(OH)2D3 and highphosphate diet were able to develop severe CKD and vascular disorder. The research provide a simple and stable mice modet to study vascular calcification in chronic kindney disease.

Key words: kidney diseases, vascular calcification, disease models, animal