Abstract: ［Abstract］〓Objective〖HTSS〗〓To construct a cDNA Library of Giardia lamblia (C2) strains.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓By extraction of C2 strains of Giardia lamblia trophozoites RNA using the Trizol method, and by using RNA as a template by reverse transcriptase cDNA single strand. dscDNA was obtained by LDPCR amplification. dscDNA was purified and co transformed with pGADT7Rec in yeast strain Y187. Through auxotrophic medium SD/Leu screening, transformants including Giardia lamblia cDNA library were obtained. Then the storage capacity and DNA recombination rate of the library were identified.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Using RNA as template, the ideal double stranded cDNA was obtained by reverse transcription, LDPCR, purification and other steps. The dscDNA and pGADT7Rec were transformed into yeast strain, and the transformants were successfully isolated and cultured. Finally, a cDNA Library of Giardia was constructed. The library capacity was 2.715×107/mL, the recombination rate was 76.7%, and the size of the inserted fragments was mainly between 300bp and 1000bp.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓An ideal C2 Library of Giardia lamblia cDNA was constructed.

Key words: Giardia, gene library, twohybrid system techniques