Abstract: ［Abstract］Objective〖HTSS〗To explore the effect of active monitoring training and simulated prevention and control training on multidrug-resistant organism(MDRO) infection in high-risk patients and to provide evidence for reducing MDRO nosocomial infection and developing scientific and effective MDRO prevention and control strategies.
〖HTH〗〖WTHZ〗Methods〖HTSS〗Simulated prevention and control training and active screening of multi-drug resistant target organisms were carried out on 222 patients in intensive care unit(ICU) who were chosen to be the experimental group from February to December in 2017. Compared with 210 patients in ICU from February to December in 2016, who were chosen to be the control group without mandatory intervention measure. The constituent ratios, the colonization rate of MDRO and the changes of contact isolation compliance were observed between two groups.
〖HTH〗〖WTHZ〗Results〖HTSS〗The nosocomial infection rate, the infection rate of MDRO in two groups,there was no significant difference between two groups(P＞0.05), there were significant differences in compliance with the MDRO isolation measures performed by medical staff such as the isolation′s advice, the bedside isolation, the isolation gown, the isolation sign, hand sanitizer and the notice among departments(P＜0.05). The experimental group was screened for escherichia coli producing extended-spectrum β-lactamase（ESBLs-ECO）, klebsiella pneumoniae producing extended-spectrum β-lactamase（ESBLs-KPN ）and multidrug-resistant Acinetobacter baumannii(MDR-AB), the colonization rates of them were 10.10%, 5.72% and 16.83% respectively. The usage rate of antibiotics were all higher than 90% and the bacterial examination rate before the use of antibiotics were all lower than 30% in two groups.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The situation of multidrug-resistant Acinetobacter baumannii colonization in ICU is quite serious, and patients with colonization are easier to obtain hospital infection. The intervention of active screening culture and simulated prevention and control training are implemented to effectively prevent and control the spread of multidrug-resistant organisms in hospitals.

Key words: multidrug-resistant organisms, active screening culture, simulated training, cross infection