Abstract: ［Abstract］ Objective〖HTSS〗To investigate the value of application of CK(AE1/AE3) by immunohistochemical staining in sentinel lymph node metastasis analysis of breast cancer patients.
〖WTHZ〗Methods〖HTSS〗A total of 1 585 sentinel lymph node tissues of breast cancer were collected. The frozen residual tissues of sentinel lymph nodes were subjected to standardized dehydration, transparent, paraffin-embedded and sectioned. Hematoxylin-eosin staining(HE) and immunohistochemical CK(AE1/AE3) markers were used to compare the difference of the two methods in sentinel lymph node metastasis screening.
〖WTHZ〗Results〖HTSS〗 Immunohistochemical staining of 1 585 sentinel lymph node tissues showed that there were 95 lymph node macrometastases, 36 lymph node micrometastasis, and 54 isolated tumour cells(ITC); The results of HE staining showed that the frozen report was 5 cases of suspicious lymph node metastasis. But immunohistochemical staining of CK(AE1/AE3) showed that there was no metastasis in 3 cases.
〖WTHZ〗Conclusion〖HTSS〗Immunohistochemical staining of CK(AE1/AE3) in sentinel lymph nodes of breast cancer patients can reduce the false positive rate of HE staining, help to detect solitary tumor cells, improve the accuracy of lymph node metastasis, thus effectively assist pathological diagnosis, and provide a basis for the selection of clinical treatment.

Key words: breast cancer, sentinel lymph node, immunohistochemical