Abstract: Objective  To detect the protein expressions of chromosome segregation like 1 protein（CSE1L） in human laryngeal squamous cell carcinoma(LSCC), and to explore the relationship between CSE1L and cervical lymph node metastasis of LSCC. 
Methods  A total of 76 specimens of human LSCC confirmed by pathology after surgery were collected, including 39 cases with cervical lymph node metastasis and 37 cases without lymph node metastasis. Another 32 specimens of adjacent normal laryngeal mucosa were collected in the meantime. The protein expression of CSE1L in tissue of LSCC and adjacent normal laryngeal mucosa was detected by immunohistochemistry and Western blot. Relationships of cervical lymph node metastasis and other clinical characteristics with CSE1L protein expression of patients were analyzed. 
Results  Among the 39 patients, the positive expression rate of CSE1L in those with cervical lymph node metastasis was 97%（38/39），including 31 patients with strong positive expression, 6 patients with moderate positive expression，1 patient with weak positive expression and only 1 patient with negative expression. Among the 37 patients who had no cervical lymph node metastasis, the positive expression rate of CSE1L was 38%（14/37），including 2 patients with strong positive expression, 11 patients with moderate positive expression，1 patient with weak positive expression and 23 patients with negative expression.  Among the 32 specimens of normal laryngeal mucosa, negative CSE1L expression was reported.  
Conclusion  CSE1L was significantly positively correlated with cervical lymph node metastasis in human LSCC. CSE1L may be a biological marker for judging cervical lymph node metastasis in human LSCC, thereby providing more reliable theoretical basis for the judgment of clinical cervical lymph node metastasis and the implementation of cervical lymph node dissection.