Abstract: Objective To study the correlation between the expression level of autophagy marker gene in peripheral blood and combinedorgan injury of patients with hemorrhagic shock. 
Methods In total, 128 hemorrhagic shock patients were selected as the hemorrhagic shock group, and 54 patients with traumatic hemorrhage but without shock were selected as the traumatic hemorrhage control group. In addition,150 healthy volunteers undergoing physical examination were selected as the healthy control group. The protein expression levels of Beclin-1 and autophagy protein 5 (Atg5) in peripheral blood of all subjectswere detected. The expression levels of Beclin-1 and Atg5 proteins between hemorrhagic shock patients with and without acute heart failure, acute liver injury, and acute kidney injury were compared. The correlation of Beclin-1, Atg5 protein with injury severity score, acute physiology and chronic health evaluation Ⅱ (APACHE-Ⅱ) score, sequential organ failure assessment (SOFA)score, N-terminal pro B-type natriuretic peptide (NT-proBNP), total bilirubin (TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum creatinine (SCr), blood urea nitrogen(BUN), left ventricular ejection fraction (LVEF)were analyzed. 
Results The protein expression levels of the autophagy marker genes Beclin-1 and Atg5 in the peripheral blood of hemorrhagic shock group were higher than those of the traumatic hemorrhage control group and healthy control group (P＜0.05). In the hemorrhagic shock group, the protein expression levels of the autophagy marker genes Beclin-1 and Atg5 in the peripheral blood of patients with heart failure, kidney injury and liver injury after admission were lower than those of patients without heart failure, kidney injury and liver injury (P＜0.05). The protein expression level of the autophagy marker genes Beclin-1 and Atg5 in the peripheral blood of patients with hemorrhagic shock at admission was negatively correlated with injury severity score, APACHE-Ⅱ score, SOFA score, NT-proBNP, TB, ALT, AST, SCr,and BUN, and positively correlated with LVEF. 
Conclusion The increased expression of autophagy marker genes in the peripheral blood of patients with hemorrhagic shock can play a compensatory protective role, which are negatively correlated with the aggravation of the disease and the degree of organ injury. 

Key words: shock, autophagy, Beclin-1, autophagy-related protein 5