Abstract: Objective  To investigate the relationship between the expression of Queuine tRNAribosyl transferase 1  (QTRT1) and the prognosis and tumor immunity in kidney renal clear cell carcinoma (KIRC) by means of bioinformatics analysis. 
Methods  The research was carried out through multiple software and online tools such as R software, TCGA database, GEO database, HPA database, STRING database, GENEMAIN database, GSEA software, TIMER2.0 database, Sanger box 3.0 online platform, and GEPIA2 platform, supplemented by qRT-PCR for cell experiment verification. 
Results  By conducting rank sum tests on 535 patients with cancer and 72 normal tissues in TCGA, it was found that the expression of QTRT1 in KIRC tissue was significantly higher than that in normal tissue (P＜0.05). The mRNA expression intensity of QTRT1 in 780-O and Caki2 of KIRC cells was significantly stronger than that in normal renal tubular epithelial cell HK2 (P＜0.05). The mRNA expression of QTRT1 showed statistical significance in different groups of T stage and clinical stage (P＜0.05). Age, grade, M stage, and QTRT1 expression were all significantly correlated with OS of KIRC. Age, grade M-stage and QTRT1 expression could serve as independent prognostic factors for OS in KIRC (P＜0.05). A total of 19 genes with the most significant interaction with QTRT1 were displayed. Simultaneously, a PPI network was constructed using the STRING platform, and 10 proteins interacting with QTRT1 were displayed. The relationship between QTRT1 and Tumor Mutation Burden (TMB) was obtained using Sangerbox, and there was a positive correlation between QTRT1 and TMB in KIRC (P＜0.05). 
Conclusion  QTRT1 is significantly upregulated in KIRC, and its upregulation is a poor prognostic signal in KIRC patients. QTRT1 is closely related to multiple immune factors such as immune cell infiltration, and immune checkpoints in KIRC, and is a potential target for immunotherapy. 

Key words: carcinoma, renal cell； QTRT1, immunity