Abstract: Objective To explore the correlation of the expression of serum sex hormone-binding globulin (SHBG) and thioredoxin interacting protein (TXNIP) with insulin resistance, and glucose and lipid metabolism in gestational diabetes mellitus (GDM). 
Methods In total, 112 GDM patients admitted to the Northwest Women's and Children's Hospital between January 2021 and January 2023 were selected as the research subjects, and another 112 pregnant women without GDM undergoing pregnancy examination in our hospital during the same period were selected as the control group. The levels of fasting blood glucose (FBG), 2-hour postprandial glucose (2 hPG), insulin resistance index (IRI), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), serum SHBG and TXNIP were compared between the two groups. The correlation of the expression of serum SHBG and TXNIP with FBG, 2 hPG, glycated hemoglobin, TG, TC, LDL-C, fasting insulin and IRI was analyzedas well as the evaluation value of serum SHBG and TXNIP in the pathogenesis of GDM. 
Results The FBG, 2 hPG, glycated hemoglobin, TG, TC, LDL-C, fasting insulin and IRI in the research group were higher than those in the control group (P＜0.05). The SHBG of the research group was lower than that of the control group, while TXNIP was higher than that of the control group (P＜0.05). Serum SHBG was negatively correlated with FBG, 2 hPG, glycated hemoglobin, TG, TC, LDL-C, fasting insulin and IRI (P＜05). Serum TXNIP was positively correlated with FBG, 2 hPG, glycated hemoglobin, TG, TC, LDL-C, fasting insulin and IRI (P＜05). IRI, glycated hemoglobin and serum TXNIP were identified as risk factors for the development of GDM (P＜05), while serum SHBG was protective factor for GDM (P＜05). The area under the curve (AUC) of serum SHBG and TXNIP alone and in combination in the assessment of development of GDM was 0.771, 0.751, and 0.844, respectively. 
Conclusion FBG, 2 h PG, glycated hemoglobin, TG, TC, LDL-C, fasting insulin and IRI are negatively correlated with serum SHBG and positively correlated with serum TXNIP. 

Key words: diabetes, gestational, insulin resistance, metabolism