Abstract: Objective To establish a reversed phase high performance liquid chromatography (RP-HPLC)method for determination of emodin and physcion in Jueming Jiangzhi Tablets. Methods RP-HPLC system was carried out on Kromasil C18(250mm × 4. 6mm,5μm)column with a mobile phase, the standards and samples were separated using a gradient mobile phase consisting of methanol, tetrahydrofuran and 1% phosphoric acid(84: 7: 9). The column temperature was 25℃ and the flow rate was 1. 0mL/ min. The nuciferine was detected at the wavelength of 254 nm. The injection volume was 20μL. Results Emodin and physcion were separated from impurities well and its retention time was at about 6. 9 min,11. 8 min. Regress equation was Y = 3. 31 × 104 X - 4. 63 × 103(R = 0. 999 7). The linear in the range of emodin was 3. 12 - 9. 36mg / L. The average recovery was 102. 1% ,and its RSD rate was 1. 7%(n = 9);Regress equation was Y = 2. 35 × 104 X + 8. 80 × 103(R = 0. 999 8). The linear in the range of physcion was 2. 1 - 10. 5mg / L. The average recovery was 104. 7% ,and its RSD rate was 1. 3%(n = 9 ). Conclusion RP-HPLC is areliable and accurate method,which can be applied for determination of emodin and physcion in Jueming Jiangzhi Tablets.

Key words: chromatography, high performance liquid, Jueming Jiangzhi Tablets, emodin