Journal of Hebei Medical University

Previous Articles     Next Articles

The experimental study of effects of Bushen Huoxue Guchi formula on BMP2 and  TRPV5/TRPV6 channels expressions in MC3T3E1 cells

  

  1. 1. DepartmentofStomatology , theHospitalofTraditionalChineseMedicine , HebeiProvince , Shijiazhuang
    050017 , China ; 2. DepartmentofStomatology , theFirstPeople'sHospitalofBeijingDongcheng
    District , Beijing 100010 , China ; 3. DepartmentofStomatology , theFirstHospitalof
    ShijiazhuangCity , HebeiProvince , Shijiazhuang 050011 , China
  • Online:2016-03-25 Published:2016-05-11

PDF (PC)

235

Abstract:
[Abstract ] Objective ToobservetheeffectofBushen HuoxueGuchiFormulaonbone
morphogeneticprotein2 ( BMP2 ) andTRPV5 / TRPV6channelexpressions , andtoexplorethe
mechanism ofBushen Huoxue GuchiFormulainboneformation.Methods Herb-contained
serum waspreparedfromratsbygavagewithBushen HuoxueGuchiFormulabyusingbody
surfaceareaconvertedequivalentdose.Blank-containedserumascontrolgroupwaspreparedby
gavagewithnormalsaline.TheBMP2andTRPV5 / TRPV6mRNAexpressionsweredetectedin
MC3T3-E1cellsby RT-PCRafterthecellsweretreated with10% herb-containedserum or
blank-containedserumfor24h , 48hand72h , respectively.Results BMP2 , TRPV6mRNA
weresignificantlyincreasedin herb-containedserum treatmentgroupcompared with blank-containedserum treatmentgroup ( P <0.05 ) .Withtheprolongation oftime , the mRNA
expressionof BMP2 and TRPV6 had a graguallyincreased tendency , butthere was no
statisticallysignificantdifference ( P >0.05 ) .TRPV5 mRNA wasnotdetectedeitherinherb-
containedserumtreatmentgrouporblank-containedserumtreatmentgroup.Conclusion Bushen
HuoxueGuchiFormulamayupregulateBMP2expressionthroughTRPV6channelstoinduce
differentiationofosteoblast.

Key words: periodontaldiseases , BushenHuoxueGuchiFormula , real-timepolymerase
chainreaction