Abstract: Objective　The present paper introduces an integrated method for stereotaxic operation on the rat brain, using three different procedures for correcting the brain targeting site.Methods　Before the ordinary method for correcting the target site ,on the stereotaxic instrument the target site was exposured and the stereotaxic coordinates were corrected in fixed brain , and then unfixed brain, respectively.Results　As the application of three methods is set up,the defect of every method can be removed and the course of experiment can be shorten,so the efficiency of the tanget site in the rat brainon stereotaxic coordinates is improved.Conclusion　With fewer animals and fewer preliminary tests the brain target site can be corrected accurately.