Abstract: ［Abstract］〓Objective〖HTSS〗〓 To explore the effects and significances of methylated modification on the biological behaviors of colorectal cancer(CRC) cell lines SW620 and LoVo in vitro.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓SW620 and LoVo were treated with SAdenosylLmethionine(SAM) for six days. Then the flow cytometry assay was used to assessed the changes of cell growth cycle and apoptosis，the transwell assay was used to detect the changs of cell invasion and migration, the plate colony assay was used to testify the changes of cell colonyformation ability, thereby to study the effects of SAM on the biological behaviors of CRC cells. SAdenosylLhomocysteine(SAH)treated cells and phosphate buffer saline(PBS)treated cells were used as control groups in the study.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with either SAHtreated cell groups or PBS treated ones, the cell cycle of SW620 cell cycle G1% and LoVo cell cycle S% in SAM group were higher than that in SAH group and PBS group(P＜005). Compared with SAH treated cells and PBS treated ones, the mean cell numbers of SW620 and LoVo treated with SAM were all significantly less than that in SAH group and PBS group through the microporous membrane or the matrigel, the difference is statistically significant(P＜005). The colonyformation rates of SW620 and LoVo treated with SAM were significantly lesser than that treated with SAH and PBS(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The exogenous methylation agent can inhibit biological behaviors of CRC cells in vitro. Methylated donor drugs are expected to be effective in the clinical treatment of CRC and provide a preliminary theoretical basis for its clinical application.

Key words: colorectal neoplasms, methylation, SAdenosyLmethionine