Journal of Hebei Medical University ›› 2021, Vol. 42 ›› Issue (6): 690-694.doi: 10.3969/j.issn.1007-3205.2021.06.015

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Correlation of abnormal methylation of the IL-12B gene promoter region with the risk of ovarian endometriosis

  

  1. 1.Department of Prevention and Health Care, the Fourth Hospital of Hebei Medical University,Shijiazhuang
    050011, China; 2.Department of Molecular Biology, the Fourth Hospital of Hebei Medical University, 
    Hebei Province, Shijiazhuang 050011, China; 3.Department of Reproductive Medicine, 
    the Fourth Hospital of Hebei Medical University, Shijiazhuang 050041, China; 
    4.Department of Gynecology, the Fourth Hospital of Hebei 
    Medical University, Shijiazhuang 050011, China
  • Online:2021-06-25 Published:2021-07-05

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Abstract: Objective  To investigate the methylation status of interleukin-12B（IL-12B） gene promoter region and its mRNA expression level in ectopic endometrium and normal endometrium, and to analyze its role in ovarian endometriosis. 
Methods  A pyrosequencing assay was used to analyse the methylation levels of IL-12B gene promoter region in ectopic endometrium of 50 patients with role in ovarian endometriosis and normal endometrium of 44 control women. The mRNA expression of IL-12B gene was detected by quantitative real-time PCR(qRT-PCR). 
Results  The methylation levels of the IL-12B gene promoter region in ectopic endometrium were significantly lower than those in normal endometrium(P＜0.001). mRNA levels of IL-12B gene were significantly increased in ectopic endometrium compared to those in normal endometrium(P＜0.001). Correlation analysis showed that the methylation levels of the IL-12B gene promoter region were negatively correlated with its mRNA levels(P＜0.001). 
Conclusion  The aberrant methylation of the IL-12B gene promoter region may play an important role in the occurrence and development of ovarian endometriosis by affecting its mRNA expression level. 


Key words: endometriosis, interleukin-12, DNA methylation