Silencing PLK1 with small interfering RNA modulates biologic behavior of lung adenocarcinoma#br#

doi:10.3969/j.issn.1007-3205.2019.11.002

Abstract

Abstract: ［Abstract］Objective〖HTSS〗To investigate the effect of down-regulation of PLK1 on the biological behavior of lung adenocarcinoma H1792 cells and analyze related mechanisms.
〖HTH〗〖WTHZ〗Methods〖HTSS〗PLK1 siRNA and control siRNA were transiently transfected into H1792 cells for 48 h respectively. Gene and protein expressions of the PLK1， E-cadherin and Vimentin were detected by real time polymerase chain reaction（PCR） and western blot assay. The cell cycle were determined by using flow cytometry. Invasion ability were evaluated by Transwell assay.
〖HTH〗〖WTHZ〗Results〖HTSS〗The expression level of PLK1 mRNA in PLK1 siRNA group was significantly lower than that in control siRNA group(P＜0.05). The expression of PLK1 protein and Vimentin protein in PLK1 siRNA group was significantly lower than that in control siRNA group, and the expression of E-cadherin protein was significantly higher than that in control siRNA group(P＜0.05). The proportion of G2/M phase cells in PLK1 siRNA group was significantly higher than that in control siRNA group, and the proportion of cells in S phase was significantly lower than that in control siRNA group(P＜0.05). The number of transmembrane cells in the siRNA group was significantly lower than that in the control siRNA group(P＜0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗PLK1 plays an important role in the growth, invasion and metastasis of lung adenocarcinoma.

Key words: lung neoplasms, rna interference, cell cycle

DENG Hao-wen1, WANG Zhi-yu2, LIU Liang3, WU Rui-feng4, LI Chao5, WANG Lei1*. Silencing PLK1 with small interfering RNA modulates biologic behavior of lung adenocarcinoma#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.1007-3205.2019.11.002.

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Silencing PLK1 with small interfering RNA modulates biologic behavior of lung adenocarcinoma#br#

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