Journal of Hebei Medical University ›› 2021, Vol. 42 ›› Issue (8): 872-875,封三.doi: 10.3969/j.issn.1007-3205.2021.08.002

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Effect of CM-Dil labeling on bone marrow stromal stem cells and its tracking in rat model of vaginal reconstruction

  

  1. 1.Department of Reproductive Medicine, the Fourth Hospital of Shijiazhuang City, Hebei Province, 
    Shijiazhuang 050011, China; 2.Department of Obstetrics and Gynecology, the Xiangya 
    Changde Hospital, Hunan Province, Changde 415000, China; 3.Department of Obstetrics 
    and Gynecology, the Second Hospital of Hebei Medical University, 
    Shijiazhuang 050000, China
  • Online:2021-08-25 Published:2021-08-27

Abstract: Objective  To explore the effect of chloromethyl-benzamidodialkylcarbocyanine(CM-Dil) labeling on bone marrow stromal stem cells(BMSCs) and its tracking in rat model of vaginal reconstruction. 
Methods  Rat BMSCs were prepared with the whole bone marrow adherent method. BMSCs were labeled with different concentrations of CM-Dil and their labeling efficiency and reproductive activities were observed. The rat model of vaginal reconstruction was established with BMSCs seeded on small intestinal submucosal matrix(SIS). Its tracking in rat model of vaginal reconstruction was observed. 
Results  BMSCs could be labeled with different concentrations of CM-Dil and the labeled efficiency was>98%, but the fluorescence intensity became stronger with the increase of concentration. The growth curves after labeling with the concentration of 2.5 μmol/L and 5 μmol/L were not significantly different from those of the control group, and the growth curves after labeling with 10 μmol/L and 20 μmol/L were slightly lower than those of the control group. After transplantation, BMSCs were mainly distributed in the deep vaginal tissues, and orange BMSCs could be found at 3 months after vaginal reconstruction. 
Conclusion  CM-Dil did not affect stem cell activity, and 5 μmol/L may be the optimal labeling concentration. Orange BMSCs could be found at 3 months after vaginal reconstruction and could be used in further study.


Key words: benzamides,  , mesenchymal stem cells, vagina